luteus is gram positive and E. Are all bacteria the same size? 4. The procedure is based on the ability of. The results showed during the conversion of milk to Kefir there was gas produced, a decrease in pH, more bacteria present, and an increase in absorbency. The only work limit is for the Abstract section (see below). Hospital Corpsman 1 & C: August 1986, Chapter 7: Clinical Laboratory. Any basic dye such as methylene blue, safranin, or crystal violet can be used to color the bacterial cells. Freshly isolated strains of N. What is important is content and correct writing style/grammar. gram staining lab report The output is a file gram staining lab report that contains all of the records that were retrieved from the target database using the input file. is a top-notch writing service that has continued to offer Gram Staining Lab Report high quality essays, research papers and coursework help to students for several years. Motivations for Starting a Small Business - Provides different motivations for starting a small business. The report is worth 100 points. A sputum Gram’s stain is a quick, low-risk laboratory test. Microbiology lab report : 1- introduction (Gram Staining) 2-Requirements (Reagents) 3-Method 4-Observations • Contents : - 3 4. Microbiology lab report on gram stain. Results cells from the Gram-stained slides. It was developed by Hans Christian Gram; a Danish physician, in 1884. Hoffmann-La Roche Ltd, Lorne Laboratories Limited, Lennox Framework Agreement, Labema Oy, Axon Lab AG and others. The Gram stain is the first test used in bacterial classification. Most living microorganisms are generally colorless and almost. Gram Staining of Bacteria from Environmental Sources. The color, size, and shape of the cells help identify the bacteria. This test differentiate the bacteria into Gram Positive and Gram Negative Bacteria, which helps in the classification and differentiations of microorganisms. Christian Gram who categorized bacteria, based on the differences in the composition of …. Amanda Ratliff Lab Report #1 3-5-12 Gram Staining Lab Report Introduction: To better understand and recognize the difference between gram positive and. LABORATORY REPORT SHEET QUESTIONS: 1. The tests used to confirm the fermentation were a gas production test, pH test, Gram stain, and turbidity test. The length of the report is inconsequential. Christian Gram in 1884, and categorizes bacteria according to their Gram character (Gram positive or Gram negative). Gram staining differentiates bacteria by allowing detection of peptidoglycan abundance. Hendrickson 13 September 2017 Purpose For the purpose of the gram staining experiment was to learn this specific type of staining technique, as well as learn how distinguish the differences between Gram positive and Gram negative stains. Characteristic Illustration; Gram stain Cell Morphology: Gram-negative diplococcus: Colony Morphology. Gram stain or Gram staining, also called Gram's method, is a method of staining used to distinguish and classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. Microbiology lab report on gram stain. Better grades, less hassle!. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. " This may mean that there is no bacterial infection present or that there were not enough bacteria present in the sample to be seen with the stain under a microscope. 16 Oct 2012 - 4 min - Uploaded by. Gram Staining Lab Report No matter Gram Staining Lab Report where you are now - even Gram Staining Lab Report if you’re relaxing now in the United Arab Emirates, our qualified specialists are around the corner to help you. Otherwise, write the report such that it is complete, regardless of the length. The name comes from. Characteristic Illustration; Gram stain Cell Morphology: Gram-negative diplococcus: Colony Morphology. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sourcesRead more about We can work on Microbiology Lab Report- Gram Staining, Simple Staining, Negative Staining[…]. Yunusa, et al. You can choose additional services like progressive delivery and professional proofreading. Good practice requires that the report on the Gram smear should mention the presence or absence of yeast cells. Filed Gram staining can be described as a staining technique used to classify bacteria which is bacteria that are stained with crystal violet followed by a brief treatment with Gram's iodine and after being decolourised with alcohol and treated with safranin then washed with water. The liquid cultures are usually examined visually, and if turbidity is present, a smear is made for Gram staining. Also, I inoculated a tryptic soy broth (TSB) with my bacterial isolate and a slant, for genomic DNA extraction and storage in the fridge, respectively. Gram-negative bacteria are a group of bacteria that do not retain the crystal violet stain (when rinsed with alcohol) used in the Gram staining method of bacterial differentiation, [1] making positive identification possible. What determines Gram-positive and Gram-negative bacteria is due to difference in cell wall composition. We use this new test in our lab to complement the results of the Gram test and not as a replacement for the commonly used Gram stain. For those who perform the Gram stain and report Gram stain results. T1 colonies predominate in the first culture of an isolate; in subsequent subcultures, T3 colonies predominate and T1 colonies may not be. Are all bacteria the same size? 4. Gram-staining was firstly introduced by Cristian Gram in 1883. performing Gram stains. Gram staining or Gram stain, also called Gram's method, is a method of staining used to differentiate bacterial species into two large groups (gram-positive and gram-negative). The Gram stain is a differential technique that is commonly used for the purposes of classifying bacteria. This easy-to-use Gram stain kit allows you to view Gram-positive bacteria (blue) versus Gram-negative bacteria (pink) for bacteriological identification. The significance of the Gram staining procedure is that it differentiates the bacteria into two groups. Positive Gram stain results usually include a description of what was seen on the slide. Gram staining depends on the structure and composition of the cell wall of bacteria. Results cells from the Gram-stained slides. marcescens are gram negative. Point to ONE bacterial cell. Streak Plate. With this staining procedure, the vegetative bacteria stain blue and the endospores are colorless. 07-14-10, lab report 10-2-10, lab report 07-7-10, lab report, Gram stain of CSF secondary to suspected diagnosis of meningitis. Lab Report A differential staining procedure used in microbiology is called the Gram stain. Laboratory Supplies Stock cultures: Bacillus cereus, Gram positive rod 1 plate/table. Gram staining or Gram stain, also called Gram's method, is a method of staining used to. The report is worth 100 points. Differentiating Organisms Using a Gram Stain. Hoffmann-La Roche Ltd, Lorne Laboratories Limited, Lennox Framework Agreement, Labema Oy, Axon Lab AG and others. The liquid cultures are usually examined visually, and if turbidity is present, a smear is made for Gram staining. After pinpointing it was a Gram positive rod, a Methyl Red-Voges Proskauer (MRVP) test was conducted as well as other testes stated in table 1. Exercise 7B Gram Staining an Unknown. 1620 words (6 pages) Essay in Biology. The timely report of a Gram stain result gives the physician important information about the presence and. Gram stain 3. In the microbiology field, extensive staining techniques are used. Many different types of both gram negative and gram positive bacteria were identified. ENDOSPORE STAIN. THE GRAM STAIN Microbiology 3051, Section 009 September 12, 2013 This report represents my individual effort. A retrospective review of patient records was performed to determine the statistical relationship between sputum Gram stain and culture results in adult men at a Veterans Affairs Medical Center in the Midwest. Experiment 2. Done in any lab. The Gram stain is a type of differential stain that allows a (micro)biologist to. The tests used to confirm the fermentation were a gas production test, pH test, Gram stain, and turbidity test. Return to Menu for Lab 7. Next, I determined if my bacterial isolate was Gram-positive or Gram-negative and used the Gram staining techniques for this process (Lab 4 protocol). The liquid cultures are usually examined visually, and if turbidity is present, a smear is made for Gram staining. Please consult your doctor or the laboratory that performed the test(s) to obtain the reference range if you do not have the lab report. This section contains your interpretation of the data that you reported in the “Results” section. Addition of iodine (mordant) forms crystal violet iodine complex within the cell wall. Christian Gram in 1884, and categorizes bacteria according to their Gram character (Gram positive or Gram negative). Wait 1 minute. It was developed by Dr. The UPHS - Marquette Reference Lab is a fully integrated, comprehensive provider of laboratory services to inpatients, outpatients, outreach clients and other community health care organizations across Michigan's Upper Peninsula. is a top-notch writing service that has continued to offer Gram Staining Lab Report high quality essays, research papers and coursework help to students for several years. ENDOSPORE STAIN. View Lab Report - The Gram Stain Lab Report from MICRO 3050 at Clemson. In this procedure living, potentially pathogenic bacteria are smeared on the glass slide and allowed to dry. Please note that the quality of the smear (too heavy or too light cell concentration) will affect the Gram Stain results. Gram-staining was firstly introduced by Cristian Gram in 1883. The results from the gram stain were the first big indicator of what unknown #72 might be. Start with simple, concise statements. In some rare cases, Gram stain and KOH test results differ. Gram Staining Lab Report Find Gram Staining Lab Report your computer and contact us 24/7 online!. Identify the cell as bacillus, coccus, or spirillium. Christian Gram in 1884 introduces Gram staining technique and classified bacteria on its Gram characters which might be Gram-negative or Gram-positive. After pinpointing it was a Gram positive rod, a Methyl Red-Voges Proskauer (MRVP) test was conducted as well as other testes stated in table 1. OVERALL FORMAT. The contrast between a cell, which is primarily water, and the background, which is water, is poor. coli have an additional outer membrane that is composed of. 10 SECs per LPF were rejected. Essay Example on Gram Staining Lab Report. Individual bacteria observed using a microscope, descriptions included, focus on a single bacterium (research)__3__2__1__0 Gram stain procedure attempted, student was able to stain and identify types of bacteria __3__2__1__0 Overall Presentation - report shows attention to detail, provides evidence from the lab, organized __3__2__1__0. The results of the Gram stain will aid a clinical lab in determining which additional tests may be required for identifying the bacterial strain in question. Record your results of staining. The name comes from. ENDOSPORE STAIN. PERFORMANCE OBJECTIVES FOR LAB 7. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed. A gram stain is a "quick look" at a sample that is suspected to be infectious. UNKNOWN IDENTIFICATION LABORATORY REPORT Introduction The Gram Stain test: This is a very important preliminary test used in initial classification as well as characterization of bacteria. Gram staining depends on the structure and composition of the cell wall of bacteria. Gram staining or Gram stain, also called Gram's method, is a method of staining used to differentiate bacterial species into two large groups (gram-positive and gram-negative). Bacteria with thin or absent cells walls are gram-negative because they do not trap the Gram stain dye. Yunusa, et al. The sample is sent to a lab. T1 colonies predominate in the first culture of an isolate; in subsequent subcultures, T3 colonies predominate and T1 colonies may not be. UNKNOWN IDENTIFICATION LABORATORY REPORT Introduction The Gram Stain test: This is a very important preliminary test used in initial classification as well as characterization of bacteria. 16 Oct 2012 - 4 min - Uploaded by. After completing this lab, the student will be able to perform the following objectives: A. They will teach you how to write precisely. Prepare a Gram stain from the NA slant. Gram staining is a microbiologic procedure used to differentiate Gram-negative from Gram-positive bacteria. • the Gram's method are commonly classified as Gram-positive and Gram negative. The Gram stain is a differential technique that is commonly used for the purposes of classifying bacteria. Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. We deliver polished, flawless grammar and composition to guarantee the academic success of That way, you're guaranteed to get the results you're looking for. The liquid cultures are usually examined visually, and if turbidity is present, a smear is made for Gram staining. This typically includes:. The gram staining procedure is the most widely recognized method utilized today to have the capacity to distinguish the diverse types of bacteria. Therefore, to see bacteria with the microscope, it is necessary to apply colour by using a staining reagent. Based on differences in. Good practice requires that the report on the Gram smear should mention the presence or absence of yeast cells. coli have an additional outer membrane that is composed of. This should be indicated on the specimen requisition. These tests and the direction of the lab instructor pointed to Bacillus cereus. The results showed during the conversion of milk to Kefir there was gas produced, a decrease in pH, more bacteria present, and an increase in absorbency. Get Your Custom Essay on Lab Report about Simple Staining of Microbes Just from $13,9/Page Get custom paper In this experiment, the microbes E. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Since everything on the slide will be the same color in simple staining, what you possibly tell about the organisms? 3. Note the long chains of rod-shaped, endospore-containing bacteria. Plates (5% sheep blood, Columbia with colistin and nalidixic acid, MacConkey and chocolate agars) were incubated for 48 h in 5% CO 2 and read after 24 h and 48 h. A Gram stain and culture of the material from an infected site are the most commonly performed microbiology tests used to identify the cause of an infection. The cells are colored red or violet so as to distinguish its group. Bacteria that stain purple with the Gram staining procedure are termed Gram-positive; those that stain pink are said to be Gram-negative. Based on your results, note the Draw Gram reaction, cell shape, and cell arrangement of each bacterium in the given table. The Gram stain is one of the oldest, most cost-efficient, yet most. Acid-fast Stain Animated Tutoria l: Another good video of the staining procedure. Put the slide, smear upwards onto the slide holder, over the sink. Experiment 2. What determines Gram-positive and Gram-negative bacteria is due to difference in cell wall composition. Many different types of both gram negative and gram positive bacteria were identified. The Gram stain is the first test used in bacterial classification. The results from the gram stain were the first big indicator of what unknown #72 might be. The color, size, and shape of the cells help identify the bacteria. 07-14-10, lab report 10-2-10, lab report 07-7-10, lab report, Gram stain of CSF secondary to suspected diagnosis of meningitis. The length of the report is inconsequential. Results: In this experiment, mixture of Bacillus cereus, Escherichia coli and. For those who perform the Gram stain and report Gram stain results. If the organism is a non-spore producing Gram negative rod and the lactose broth shows an acid/gas reaction, this is a positive completed test. With this staining procedure, the vegetative bacteria stain blue and the endospores are colorless. Point to ONE bacterial cell. Only premium essay tutoring can help you in attaining desired results. experiment explained about the principles of gram stain method and allowed the interpretation of each of the steps in gram staining. The Gram stain is a type of differential stain that allows a (micro)biologist to. Since inception, we have amassed top talent through rigorous recruiting process in addition to using sophisticated design and tools in order to deliver the best results. This section contains your interpretation of the data that you reported in the “Results” section. The thing is gram negative and gram positive stain distinctively with this procedure for a couple of reasons. Once stained, the bacteria may be observed and. gram stain lab report They are long-term assignments, and if schedules gram stain lab report are not carefully planned, students can find themselves in a panic, with papers due and not enough time to do them well. Lab Exercise 7: The Negative Stain and Capsule Stain OBJECTIVES 1. Please consult your doctor or the laboratory that performed the test(s) to obtain the reference range if you do not have the lab report. • Results of review added to lab work card and patient report. This is called a smear. After a series of differential tests, a chart of bacteria was referenced to discover the unknown bacteria was E. The sample is sent to a lab. Identify the cell as bacillus, coccus, or spirillium. Flood air-dried, heat-fixed smear of cells for 1 minute with crystal violet staining reagent. An inoculating loop was sterilized, a sample of Unknown “B” was collected and a streak was made on the agar plate. A retrospective review of patient records was performed to determine the statistical relationship between sputum Gram stain and culture results in adult men at a Veterans Affairs Medical Center in the Midwest. That is why we have introduced a long list of guarantees to protect Gram Stain Lab Report them from spending money in vain. How a Gram Stain Is Performed. Image 3: Procedure of Gram Staining; note the color change after each step. 6 From our observation from simple staining of Bacillus sp ,it can be seen that the bacteria. Only premium essay tutoring can help you in attaining desired results. Gram staining or Gram stain, also called Gram's method, is a method of staining used to differentiate bacterial species into two large groups (gram-positive and gram-negative). 2,12–15 The American Society for Microbiology. Filed Gram staining can be described as a staining technique used to classify bacteria which is bacteria that are stained with crystal violet followed by a brief treatment with Gram's iodine and after being decolourised with alcohol and treated with safranin then washed with water. Christian Gram in 1884 introduces Gram staining technique and classified bacteria on its Gram characters which might be Gram-negative or Gram-positive. Pure Culture Methods and Gram Staining. Bacteria Sampling (formerly Disposable Lab Equipment) should be followed by Gram Staining, then Using a Microscope This work was supported by USDA CSREES and USDA National Institute of Food and Agriculture under two Higher Education Challenge Grant projects: 2008-38411-19055 and 2011-38411-30625. We are offering quick essay tutoring services round the clock. Good practice requires that the report on the Gram smear should mention the presence or absence of yeast cells. Lab Report A differential staining procedure used in microbiology is called the Gram stain. Amanda Ratliff Lab Report #1 3-5-12 Gram Staining Lab Report Introduction: To better understand and recognize the difference between gram positive and. Gram Staining Procedure/Protocol: Flood air-dried, heat-fixed smear of cells for 1 minute with crystal violet staining reagent. The cells are colored red or violet so as to distinguish its group. Results cells from the Gram-stained slides. Since the unknown bacteria was a gram negative rod, a gelatin test was performed and the organism was inoculated into a gelatin agar tube and placed into the incubator. Materials: 1 exercise 7b gram staining lab report proficiency proficient not really a lab. Laboratory Supplies Stock cultures: Bacillus cereus, Gram positive rod 1 plate/table. Include a drawing of your Gram stain. Method: Refer to lab manual page 32-36. The inoculum is streaked over the agar surface to isolate colonies on at least a portion of the plate. There were even a few gram insensitive fellows that had some interesting characteristics such as being spiral shaped. The information was reported to the Professor and the correct information verified that Staphylococcus Epidermidis was in fact correct. performing Gram stains. Kit includes bacterial cultures that represent all 3 bacterial morphologies (rods, spheres, and spirals) so students can use classification to identify the bacteria. The Gram stain is a type of differential stain that allows a (micro)biologist to. Instead Gram Stain Lab Report of wasting time on amateur tutors, hire experienced essay tutors Gram Stain Lab Report for proper guidance. View Lab Report - The Gram Stain Lab Report from MICRO 305 at Clemson University. this is a negative completed test. Microbiology - Lecture notes - 1. A report of “presumptive N. In the microbiology field, extensive staining techniques are used. Report abuse. Acid-fast Stain Animated Tutoria l: Another good video of the staining procedure. First, primary stain, crystal violet stains all the cells purple. The timely report of a Gram stain result gives the physician important information about the presence and. The Gram stain has a broad staining spectrum (2, 3) and classifies bacteria as either gram positive or gram negative. Being Gram-negative bacteria, E. Procedure Purpose Reagents Results. Gram-staining was firstly introduced by Cristian Gram in 1883. Image 3: Procedure of Gram Staining; note the color change after each step. Filed Gram staining can be described as a staining technique used to classify bacteria which is bacteria that are stained with crystal violet followed by a brief treatment with Gram's iodine and after being decolourised with alcohol and treated with safranin then washed with water. The Endospore. Unknown Lab Report: Unknown “C” Discussion/Results. Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. Gram Staining Lab Report No matter Gram Staining Lab Report where you are now - even Gram Staining Lab Report if you’re relaxing now in the United Arab Emirates, our qualified specialists are around the corner to help you. Materials: 1 exercise 7b gram staining lab report proficiency proficient not really a lab. gonorrhoeae exhibit at least two of four colony forms, designated T1, T2, T3, and T4. Freshly isolated strains of N. The name comes from the Danish bacteriologist Hans Christian Gram, who developed the technique. Gram positive bacteria (which show up purple after the gram staining process) have a very thick …. T1 colonies predominate in the first culture of an isolate; in subsequent subcultures, T3 colonies predominate and T1 colonies may not be. Learn vocabulary, terms, and more with flashcards, games, and other study tools. In the microbiology field, extensive staining techniques are used. PERFORMANCE OBJECTIVES FOR LAB 7. Microbiology - Lecture notes - 1. The Procedure Of Negative Staining Technique Take a clean, Dry, Scratch and Grease free Microscopic glass slide and place a drop of India ink or Nigrosin on it at one end near the edge. com makes it easy to get the grade you want!. Good practice requires that the report on the Gram smear should mention the presence or absence of yeast cells. Some of the players in the Gram Staining Systems market includes Hardy Diagnostics, ELITechGroup, bioMérieux SA, F. If something is wrong with your order, our support team will Gram Stain Lab Report help you. Lab Report about Simple Staining of Microbes. Gram Staining Procedure/Protocol: Flood air-dried, heat-fixed smear of cells for 1 minute with crystal violet staining reagent. Since inception, we have amassed top talent through rigorous recruiting process in addition to using sophisticated design and tools in order to deliver the best results. Putting the gram stain and culture report together. This is a caption for compliance. Read through the Case. We deliver polished, flawless grammar and composition to guarantee the academic success of That way, you're guaranteed to get the results you're looking for. BLOOD GROUPING, BLOOD STAINING, AND HEMATOCRIT Laboratory Report 06 March Introduction The ABO blood grouping entails the categorisation of human blood depending on heritable traits of red blood cells as established by the existence or absence of antigens (Rogers, 2011). Gram Stain Results Purpose: To differentiate between Gram-positive and Gram-negative organisms, while simultaneously learning about the cellular morphology and arrangement. Microbiology lab report on gram stain. Start studying Lab Report 14 (Gram Staining). Logistics Lab Testing Sections: Microbiology Phone Numbers: MIN Lab: 612 -813 5866 STP Lab: 651-220-6555 Test Availability: Daily, 24 hours Turnaround Time: Preliminary report available at 1 day, final report within 2 - 5 days. DISCUSSION. The Gram stain is a classical and important staining technique that remains widely used by environmental microbiologists. The Gram stain is a type of differential stain that allows a (micro)biologist to. The preparation and analysis of a Gram stain is a highly complex procedure that requires extensive experience in order for a correct result to be reported ( 11 ). Gram Staining Lab Report Find Gram Staining Lab Report your computer and contact us 24/7 online!. 11 Recent studies have examined the impact of reporting Gram stain results on patient mortality. The procedure is based on the ability of. Gram stain 3. All bacteria have cell walls made of peptidoglycan. Procedure Purpose Reagents Results. 12 Summary - Final exam study guide for microbiology lab Exam Spring 2016, questions and answers Exam Spring 2016, questions and answers BIO 315 Viruses Summary Unknown Bacteria Lab Report. Kit includes bacterial cultures that represent all 3 bacterial morphologies (rods, spheres, and spirals) so students can use classification to identify the bacteria. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The staining technique distinguishes between two main types of bacteria (gram positive and gram negative) by imparting color on the cells. That is why we have introduced a long list of guarantees to protect Gram Stain Lab Report them from spending money in vain. gram-positive bacteria. Christian Gram in 1884 introduces Gram staining technique and classified bacteria on its Gram characters which might be Gram-negative or Gram-positive. Unknown Lab Report: Unknown “C” Discussion/Results. 1620 words (6 pages) Essay in Biology. Gram Staining Lab Report, example deans list team member essay, ucf college essay example, thesis statements workshe GrabMyEssay. Are all bacteria the same size? 4. Take a small portion of the bacterial colony with the help of sterilized Inoculating loop. Quickly memorize the terms, phrases and much more. Image 3: Procedure of Gram Staining; note the color change after each step. luteus and E. Bacteria that stain purple with the Gram staining procedure are termed Gram-positive; those that. UNKNOWN IDENTIFICATION LABORATORY REPORT Introduction The Gram Stain test: This is a very important preliminary test used in initial classification as well as characterization of bacteria. Also, your Lab Report #7 (Anaerobes) will be due at the beginning of the Final. Prior experience with Gram's stain on. Principles. " This may mean that there is no bacterial infection present or that there were not enough bacteria present in the sample to be seen with the stain under a microscope. UNKNOWN IDENTIFICATION LABORATORY REPORT Introduction The Gram Stain test: This is a very important preliminary test used in initial classification as well as characterization of bacteria. Also, I inoculated a tryptic soy broth (TSB) with my bacterial isolate and a slant, for genomic DNA extraction and storage in the fridge, respectively. After pinpointing it was a Gram positive rod, a Methyl Red-Voges Proskauer (MRVP) test was conducted as well as other testes stated in table 1. Introduction : 4 • The Gram staining • The Gram staining method, named after Hans Christian Gram. The same sample can be used for the gram stain, culture, and sensitivity. Hoffmann-La Roche Ltd, Lorne Laboratories Limited, Lennox Framework Agreement, Labema Oy, Axon Lab AG and others. The length of the report is inconsequential. The results showed during the conversion of milk to Kefir there was gas produced, a decrease in pH, more bacteria present, and an increase in absorbency. HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY. Essay Example on Gram Staining Lab Report. Done in any lab. The thing is gram negative and gram positive stain distinctively with this procedure for a couple of reasons. For example: Gram staining of sample 1A showed 3 organisms: a Gram negative diplococcus, a Gram positive streptobacillus, and a Gram positive staphylococcus. Gram Staining Formal Lab Report Miranda King Intro Microbiology Dr. The results from the gram stain were the first big indicator of what unknown #72 might be. Gram Stain Lab Report, analytical essay on oedipus the king, cosmetology research paper, for writers of term papers theses and dissertations. Positive Gram stain results usually include a description of what was seen on the slide. Kit includes bacterial cultures that represent all 3 bacterial morphologies (rods, spheres, and spirals) so students can use classification to identify the bacteria. You can charge your money back before releasing them to Gram Stain Lab Report the writer. BLOOD GROUPING, BLOOD STAINING, AND HEMATOCRIT Laboratory Report 06 March Introduction The ABO blood grouping entails the categorisation of human blood depending on heritable traits of red blood cells as established by the existence or absence of antigens (Rogers, 2011). Bacteria Sampling (formerly Disposable Lab Equipment) should be followed by Gram Staining, then Using a Microscope This work was supported by USDA CSREES and USDA National Institute of Food and Agriculture under two Higher Education Challenge Grant projects: 2008-38411-19055 and 2011-38411-30625. Return to Menu for Lab 7. View Lab Report - The Gram Stain Lab Report from MICRO 305 at Clemson University. experiment explained about the principles of gram stain method and allowed the interpretation of each of the steps in gram staining. A common occurrence is to observe organisms present in the Gram stain (e. GENERAL MICROBIOLOGY 2210: GRAM STAIN REPORT (15 PTS) ASSIGNMENT Each student will have the opportunity to submit one example of a Gram stain with a brief written report describing the staining procedure and the stain itself. The Lab Report Template is what the students use to develop the lab report for this project. Gram Staining Procedure/Protocol: Flood air-dried, heat-fixed smear of cells for 1 minute with crystal violet staining reagent. Bacteria that stain purple with the Gram staining procedure are termed Gram-positive; those that. Using a pipette, cover the slide with crystal violet stain and leave for about a minute. This method is used to distinguish between gram positive and gram-negative bacteria which have consistent differences in their cell walls. 6 From our observation from simple staining of Bacillus sp ,it can be seen that the bacteria. The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria. The Gram staining technique is the most important and widely used microbiological differential staining technique. Gram stain 3. Some of the players in the Gram Staining Systems market includes Hardy Diagnostics, ELITechGroup, bioMérieux SA, F. The results from the gram stain were the first big indicator of what unknown #72 might be. gram-positive bacteria. The expert essay Gram Staining Lab Report tutors at Nascent Minds will elaborate every single detail to Gram Staining Lab Report you. The results may have been incorrect due to a lack of growth within the Urea Broth tube, possible contamination, or simply poorly conducted. The length of the report is inconsequential. The Gram stain is the most widely used staining procedure in bacteriology. Unknown Lab Report: Unknown “C” Discussion/Results. PERFORMANCE OBJECTIVES FOR LAB 7. Following each step three times in the lab manual on how to perform a gram stain, the bacteria turned out to be gram negative rod. Good practice requires that the report on the Gram smear should mention the presence or absence of yeast cells. The cells are colored red or violet so as to distinguish its group. Gram positive bacteria simply have more peptidoglycan than gram negative bacteria. Gram stain results from 1996 were statistically compared with culture results for 2,105 sputum specimens. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Hospital Corpsman 1 & C: August 1986, Chapter 7: Clinical Laboratory. Why heat-fix the. A common occurrence is to observe organisms present in the Gram stain (e. this is a negative completed test. DISCUSSION. This method is used to distinguish between gram positive and gram-negative bacteria which have consistent differences in their cell walls. Some of the players in the Gram Staining Systems market includes Hardy Diagnostics, ELITechGroup, bioMérieux SA, F. In some rare cases, Gram stain and KOH test results differ. Please consult your doctor or the laboratory that performed the test(s) to obtain the reference range if you do not have the lab report. You can choose additional services like progressive delivery and professional proofreading. The Gram stain is one of the oldest, most cost-efficient, yet most. Gram stain or Gram staining, also called Gram's method, is a method of staining used to distinguish and classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. Otherwise, write the report such that it is complete, regardless of the length. Title: Gram Stein Technique. The Gram stain is one of the oldest, most cost-efficient, yet most. The Gram stain is the first test used in bacterial classification. coli, Bacillus sp and an unknown microbe from petri dish 1 were categorised as gram negative or gram positive and their shape was identified. Gram-staining was firstly introduced by Cristian Gram in 1883. Include a drawing of your Gram stain. A retrospective review of patient records was performed to determine the statistical relationship between sputum Gram stain and culture results in adult men at a Veterans Affairs Medical Center in the Midwest. coli Staphylococcus 2. Gram staining is a method of differentiating bacterial species (gram-positive and gram-negative). In accumulation, these staining techniques also help in the determination of cell size, structure. Gram Staining Lab Report, examples of introductory paragraphs for persuasive essays, example of biography essay about yourself, introduction paragraph essay outline. Gram-negative bacteria are a group of bacteria that do not retain the crystal violet stain (when rinsed with alcohol) used in the Gram staining method of bacterial differentiation, [1] making positive identification possible. We are offering quick essay tutoring services round the clock. Gram stain of tissue biopsy test involves using crystal violet stain to test a sample of tissue taken from a biopsy. Some of the players in the Gram Staining Systems market includes Hardy Diagnostics, ELITechGroup, bioMérieux SA, F. characterize the Gram stain reaction to a large extent. Results cells from the Gram-stained slides. • the Gram's method are commonly classified as Gram-positive and Gram negative. Laboratory perspective of gram staining and its significance in investigations of infectious diseases Yunusa Thairu 1, Idris Abdullahi Nasir 1, Yahaya Usman 2 1 Department of Medical Microbiology, University of Abuja Teaching Hospital, Gwagwalada, Abuja, Nigeria 2 Department of Medical Laboratory Science, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna, Nigeria. Often, detecting the presence of microorganisms and determining whether an infection is caused by an organism that is Gram positive or Gram negative will be sufficient to allow a doctor to prescribe treatment with an appropriate empiric. In this procedure living, potentially pathogenic bacteria are smeared on the glass slide and allowed to dry. The Gram stain is a classical and important staining technique that remains widely used by environmental microbiologists. A common method for the isolation of a pure culture from a mixture is by "streaking" plates. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. Gram Staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by Danish Bacteriologist Hans Christian Gram in 1884. Gram staining is a microbiologic procedure used to differentiate Gram-negative from Gram-positive bacteria. Hoffmann-La Roche Ltd, Lorne Laboratories Limited, Lennox Framework Agreement, Labema Oy, Axon Lab AG and others. This makes the Gram stain a valuable diagnostic tool for a medical clinic or lab. Amanda Ratliff Lab Report #1 3-5-12 Gram Staining Lab Report Introduction: To better understand and recognize the difference between gram positive and. It is called a differential stain since it differentiates between Gram-positive and Gram-negative bacteria. LABORATORY REPORT SHEET QUESTIONS: 1. Note the long chains of rod-shaped, endospore-containing bacteria. There were even a few gram insensitive fellows that had some interesting characteristics such as being spiral shaped. For these reasons, you will not find reference ranges for the majority of tests described on this web site. 16 Oct 2012 - 4 min - Uploaded by. Name two endospore-producing genera of bacteria. gram staining lab report The output is a file gram staining lab report that contains all of the records that were retrieved from the target database using the input file. • Results of review added to lab work card and patient report. UNKNOWN IDENTIFICATION LABORATORY REPORT Introduction The Gram Stain test: This is a very important preliminary test used in initial classification as well as characterization of bacteria. Essay Example on Gram Staining Lab Report. Using a second pipette, gently wash off the crystal violet with Lugnol’s iodine. Gram Staining Lab Report, example deans list team member essay, ucf college essay example, thesis statements workshe GrabMyEssay. Include a drawing of your Gram stain. Also, your Lab Report #7 (Anaerobes) will be due at the beginning of the Final. Identify the cell as bacillus, coccus, or spirillium. The Gram stain method can be used on almost any specimen. Bacteria with thick cells walls are termed gram-positive because they're susceptible to dying during a test called the Gram stain. Lab Report: No formal report: Gram Stain Results, Antibiotic sensitivity results, Bacterial plate counts and calculations are requried. Gram Staining. Most have cell membranes and cell walls. : Laboratory perspective of Gram staining and its signi fi cance in investigations of infectious diseases 173 Sub-Saharan African Journal of Medicine /Vol 1/Issue 4/Oct-Dec 2014 d. Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. • Results of review added to lab work card and patient report. The stain revealed pink, rod-shaped bacteria, indicating that the bacteria were gram negative (meaning that they had a thin layer of peptidoglycan sandwiched between two membranes in their cell wall) and bacillus. The Gram stain is a classical and important staining technique that remains widely used by environmental microbiologists. The timely report of a Gram stain result gives the physician important information about the presence and. The UPHS - Marquette Reference Lab is a fully integrated, comprehensive provider of laboratory services to inpatients, outpatients, outreach clients and other community health care organizations across Michigan's Upper Peninsula. Hendrickson 13 September 2017 Purpose For the purpose of the gram staining experiment was to learn this specific type of staining technique, as well as learn how distinguish the differences between Gram positive and Gram negative stains. Gram staining depends on the structure and composition of the cell wall of bacteria. PERFORMANCE OBJECTIVES FOR LAB 7. The contrast between a cell, which is primarily water, and the background, which is water, is poor. The lab report containing your test results should include the relevant reference range for your test(s). , Gram-positive bacilli, Gram-positive cocci, or Gram-negative bacilli) and to report these. this is a negative completed test. In particular, the presence of disease-causing. Understand the benefits of using negative stains. This typically includes:. The Gram stain is a differential technique that is commonly used for the purposes of classifying bacteria. is a top-notch writing service that has continued to offer Gram Staining Lab Report high quality essays, research papers and coursework help to students for several years. Most living microorganisms are generally colorless and almost. The procedure is based on the ability of. What is Gram Staining: Dr. Bacteria that are non-responsive to this technique are known as gram insensitive. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed. Gram stain results from 1996 were statistically compared with culture results for 2,105 sputum specimens. Gram positive bacteria (with a thicker peptidoglycan layer) retain crystal violet stain during the decolourisation process, while Gram negative bacteria lose the crystal violet stain and are instead stained by the safranin in the final staining process. The Gram staining technique is the most important and widely used microbiological differential staining technique. Since inception, we have amassed top talent through rigorous recruiting process in addition to using sophisticated design and tools in order to deliver the best results. Gram stain or Gram staining, also called Gram's method, is a method of staining used to distinguish and classify bacterial species into two large groups: gram-positive bacteria and gram-negative bacteria. laboratory report on their efforts related to staining their Gram-Stain Unknown (Skills Test 2). Bacteria that stain purple with the Gram staining procedure are termed Gram-positive; those that stain pink are said to be Gram-negative. The gram stain is used to differentiate bacteria into two groups based on cell color after staining. Point to ONE bacterial cell. In particular, the presence of disease-causing. and label each bacterial species. For these reasons, you will not find reference ranges for the majority of tests described on this web site. The Gram stain is a type of differential stain that allows a (micro)biologist to. A negative result for BV on a cervical smear could lead to inappropriate patient management. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed. 16 Oct 2012 - 4 min - Uploaded by. Principles. Experiment 2. Read through the Case. Most have cell membranes and cell walls. luteus is gram positive and E. ADVERTISEMENTS: Read this article to learn about the experiment to perform gram staining of bacteria to find out whether it is gram-positive or gram-negative! Purpose: The most important differential staining used in microbiology is gram staining. Test Includes: Culture for aerobic flora and Gram stain to assess specimen quality. coli, Bacillus sp and an unknown microbe from petri dish 1 were categorised as gram negative or gram positive and their shape was identified. Laboratory Report 15 15 Gram Staining A. Prepare a Gram stain from the NA slant. It gives relatively quick, preliminary results as to whether microbes are present and, if so, the general type(s) causing an infection. Any basic dye such as methylene blue, safranin, or crystal violet can be used to color the bacterial cells. This is an extremely useful tutorial that shows, step-by-step, the Gram-staining procedure and the appearance of Gram+ and Gram- bacterial cells. We use this new test in our lab to complement the results of the Gram test and not as a replacement for the commonly used Gram stain. Otherwise, write the report such that it is complete, regardless of the length. Introduction : 4 • The Gram staining • The Gram staining method, named after Hans Christian Gram. The Gram stain is a differential technique that is commonly used for the purposes of classifying bacteria. A negative Gram stain is often reported as "no organism seen. Gram staining is a differential staining method which results in the classification of two groups of bacteria, Gram-positive and Gram-negative. HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY. Christian Gram in 1884 introduces Gram staining technique and classified bacteria on its Gram characters which might be Gram-negative or Gram-positive. 16 Oct 2012 - 4 min - Uploaded by. Perform a negative staining procedure 2. The only work limit is for the Abstract section (see below). MCB 3020L Lab Experiment 2 The Gram Stain A one lab session experiment In most microbiological staining procedures, the bacteria are first fixed to the slide by the heat fixed smear. '' This staining response is based on the chemical and structural makeup of the cell walls of both varieties of bacteria. ADVERTISEMENTS: Read this article to learn about the experiment to perform gram staining of bacteria to find out whether it is gram-positive or gram-negative! Purpose: The most important differential staining used in microbiology is gram staining. Gram positive bacteria have thicker peptidoglycan layer than gram negative bacteria. Start studying Micro Lab - Report #15 - Gram Staining. Christian Gram in 1884, and categorizes bacteria according to their Gram character (Gram positive or Gram negative). The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria. Lab Report 1 Exercise 7B Gram Staining an Unknown. It is called a differential stain since it differentiates between Gram-positive and Gram-negative bacteria. A gram stain is a "quick look" at a sample that is suspected to be infectious. We are offering quick essay tutoring services round the clock. INTRODUCTION As mentioned in lab exercise 6, not all dyes stain the bacterial cell. The Endospore. Hendrickson 13 September 2017 Purpose For the purpose of the gram staining experiment was to learn this specific type of staining technique, as well as learn how distinguish the differences between Gram positive and Gram negative stains. Unknown Gram – Bacteria (B) was more of a challenge. The significance of the Gram staining procedure is that it differentiates the bacteria into two groups. Sputum Gram Stain. This method is used to distinguish between gram positive and gram-negative bacteria which have consistent differences in their cell walls. Freshly isolated strains of N. The reason for staining bacteria is due to the fact that most bacteria are transparent and cannot be seen through the microscope. Individual bacteria observed using a microscope, descriptions included, focus on a single bacterium (research)__3__2__1__0 Gram stain procedure attempted, student was able to stain and identify types of bacteria __3__2__1__0 Overall Presentation - report shows attention to detail, provides evidence from the lab, organized __3__2__1__0. Start with simple, concise statements. Learn vocabulary, terms, and more with flashcards, games, and other study tools. A report of “presumptive N. Lab Report A differential staining procedure used in microbiology is called the Gram stain. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Gram staining is a microbiologic procedure used to differentiate Gram-negative from Gram-positive bacteria. Because the cell walls of Gram-negative cells have a higher content of lipids and a thinner layer of peptidoglycan, the alcohol used in the decolorizing step made the Gram-negative cells incapable of retaining the methylene blue-iodine complex. The inoculum is streaked over the agar surface to isolate colonies on at least a portion of the plate. On subculture, the organisms do not grow. For those who perform the Gram stain and report Gram stain results. Gram Staining Systems Market Treatment Market: Key Players. Principles. The expert essay Gram Staining Lab Report tutors at Nascent Minds will elaborate every single detail to Gram Staining Lab Report you. Gram stain results from 1996 were statistically compared with culture results for 2,105 sputum specimens. Individual bacteria observed using a microscope, descriptions included, focus on a single bacterium (research)__3__2__1__0 Gram stain procedure attempted, student was able to stain and identify types of bacteria __3__2__1__0 Overall Presentation - report shows attention to detail, provides evidence from the lab, organized __3__2__1__0. What is important is content and correct writing style/grammar. Following each step three times in the lab manual on how to perform a gram stain, the bacteria turned out to be gram negative rod. Kit includes bacterial cultures that represent all 3 bacterial morphologies (rods, spheres, and spirals) so students can use classification to identify the bacteria. Principle: Gram-positive cells have a thick peptidoglycan cell wall that is able to retain the crystal violet-iodine complex that occurs during staining, while Gram-negative. Based on your results, note the Draw Gram reaction, cell shape, and cell arrangement of each bacterium in the given table. The results of the Gram stain will aid a clinical lab in determining which additional tests may be required for identifying the bacterial strain in question. Report abuse. It is a technique used to differentiate types of bacteria using certain physical and chemical characteristics Of their cell walls. Hoffmann-La Roche Ltd, Lorne Laboratories Limited, Lennox Framework Agreement, Labema Oy, Axon Lab AG and others. Report a wide range of laboratory diagnoses of causative agents to PHE and many. Wait 1 minute. Introduction : 4 • The Gram staining • The Gram staining method, named after Hans Christian Gram. Gram Staining Lab Introduction Gram staining is a very important technique used in biology labs all over the world. It is called a differential stain since it differentiates between Gram-positive and Gram-negative bacteria. Please note that the quality of the smear (too heavy or too light cell concentration) will affect the Gram Stain results. Quickly memorize the terms, phrases and much more. View Lab Report - The Gram Stain Lab Report from MICRO 3050 at Clemson. On what part of the cell do the antibiotics usually work? List one or two antibiotics that affect Gram-negative bacteria? On what part of the cell do the antibiotics usually work? (Be sure to cite your sourcesRead more about We can work on Microbiology Lab Report- Gram Staining, Simple Staining, Negative Staining[…]. Gram Staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by Danish Bacteriologist Hans Christian Gram in 1884. Gram staining is a differential staining method which results in the classification of two groups of bacteria, Gram-positive and Gram-negative. Fermentation Lab Report. Bacteria that stain purple with the Gram staining procedure are termed Gram-positive; those that stain pink are said to be Gram-negative. Record your results of staining. Because the cell walls of Gram-negative cells have a higher content of lipids and a thinner layer of peptidoglycan, the alcohol used in the decolorizing step made the Gram-negative cells incapable of retaining the methylene blue-iodine complex. gram staining lab report The output is a file gram staining lab report that contains all of the records that were retrieved from the target database using the input file. Next, I determined if my bacterial isolate was Gram-positive or Gram-negative and used the Gram staining techniques for this process (Lab 4 protocol). The contrast between a cell, which is primarily water, and the background, which is water, is poor. Most have cell membranes and cell walls. Individual bacteria observed using a microscope, descriptions included, focus on a single bacterium (research)__3__2__1__0 Gram stain procedure attempted, student was able to stain and identify types of bacteria __3__2__1__0 Overall Presentation - report shows attention to detail, provides evidence from the lab, organized __3__2__1__0. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed. These are the sources and citations used to research Microbiology gram staining lab report. Perform a negative staining procedure 2. Are all bacteria the same size? 4. Record your results of staining. The lab team member places a very thin layer of the sample onto a glass slide. When you submit our work, you can be confident that it is ready to hand in to your teacher or professor. We are offering quick essay tutoring services round the clock. Principles. Using a pipette, cover the slide with crystal violet stain and leave for about a minute. Gram Staining Lab Report Find Gram Staining Lab Report your computer and contact us 24/7 online!. The Gram stain is a differential technique that is commonly used for the purposes of classifying bacteria. Gram-staining was firstly introduced by Cristian Gram in 1883. Gram Staining is the common, important, and most used differential staining techniques in microbiology, which was introduced by Danish Bacteriologist Hans Christian Gram in 1884. DISCUSSION. What names are given to the shapes seen in the 2 bacteria used in lab? 5. Gram Staining. Gram staining differentiates bacteria by allowing detection of peptidoglycan abundance. This typically includes:. Addition of iodine (mordant) forms crystal violet iodine complex within the cell wall. The gram stain is used to differentiate bacteria into two groups based on cell color after staining. LAB 2: Staining and Streaking Protocols for Simple stain, Gram Stain, Streak Plate Technique and Culture Maintenance Lab 2a: INTRODUCTION To Staining Live specimens are difficult to see with the bright field microscope. Instead Gram Stain Lab Report of wasting time on amateur tutors, hire experienced essay tutors Gram Stain Lab Report for proper guidance. They will teach you how to write precisely. Laboratory perspective of gram staining and its significance in investigations of infectious diseases Yunusa Thairu 1, Idris Abdullahi Nasir 1, Yahaya Usman 2 1 Department of Medical Microbiology, University of Abuja Teaching Hospital, Gwagwalada, Abuja, Nigeria 2 Department of Medical Laboratory Science, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna, Nigeria. On subculture, the organisms do not grow. A good report will likely necessitate a minimum of 2 pages double-spaced and is limited to a 3-page maximum. Gram staining is a microbiologic procedure used to differentiate Gram-negative from Gram-positive bacteria. These are the sources and citations used to research Microbiology gram staining lab report. The process begins by obtaining a sample. The only work limit is for the Abstract section (see below). After completing this lab, the student will be able to perform the following objectives: A. Gram positive bacteria simply have more peptidoglycan than gram negative bacteria. characterize the Gram stain reaction to a large extent. Logistics Lab Testing Sections: Microbiology Phone Numbers: MIN Lab: 612 -813 5866 STP Lab: 651-220-6555 Test Availability: Daily, 24 hours Turnaround Time: Preliminary report available at 1 day, final report within 2 - 5 days. A negative result for BV on a cervical smear could lead to inappropriate patient management. Many types of samples can be tested including exudate or pus from a wound, sputum, blood, stool, or a urine sample. Since the unknown bacteria was a gram negative rod, a gelatin test was performed and the organism was inoculated into a gelatin agar tube and placed into the incubator. You can choose additional services like progressive delivery and professional proofreading. gram-positive bacteria. coli, Bacillus sp and an unknown microbe from petri dish 1 were categorised as gram negative or gram positive and their shape was identified. Gram positive bacteria have thicker peptidoglycan layer than gram negative bacteria. The Gram stain is a type of differential stain that allows a (micro)biologist to. Lab Report 1 Exercise 7B Gram Staining an Unknown. View Lab Report - The Gram Stain Lab Report from MICRO 3050 at Clemson. All bacteria have cell walls made of peptidoglycan. The staining technique distinguishes between two main types of bacteria (gram positive and gram negative) by imparting color on the cells. The significance of the Gram staining procedure is that it differentiates the bacteria into two groups. Gram Staining Lab Report Find Gram Staining Lab Report your computer and contact us 24/7 online!. 16 Oct 2012 - 4 min - Uploaded by. These are the sources and citations used to research Microbiology gram staining lab report. BLOOD GROUPING, BLOOD STAINING, AND HEMATOCRIT Laboratory Report 06 March Introduction The ABO blood grouping entails the categorisation of human blood depending on heritable traits of red blood cells as established by the existence or absence of antigens (Rogers, 2011). The cell types differ in. The results showed during the conversion of milk to Kefir there was gas produced, a decrease in pH, more bacteria present, and an increase in absorbency. Laboratory perspective of gram staining and its significance in investigations of infectious diseases Yunusa Thairu 1, Idris Abdullahi Nasir 1, Yahaya Usman 2 1 Department of Medical Microbiology, University of Abuja Teaching Hospital, Gwagwalada, Abuja, Nigeria 2 Department of Medical Laboratory Science, Faculty of Medicine, Ahmadu Bello University, Zaria, Kaduna, Nigeria. If the organism is a non-spore producing Gram negative rod and the lactose broth shows an acid/gas reaction, this is a positive completed test. It is used to differentiate bacteria based on staining differences in biochemical and physical characteristics of bacterial cell walls. Gram staining or Gram stain, also called Gram's method, is a method of staining used to. The timely report of a Gram stain result gives the physician important information about the presence and. Principle: Gram-positive cells have a thick peptidoglycan cell wall that is able to retain the crystal violet-iodine complex that occurs during staining, while Gram-negative. and label each bacterial species. Amanda Ratliff Lab Report #1 3-5-12 Gram Staining Lab Report Introduction: To better understand and recognize the difference between gram positive and. laboratory report on their efforts related to staining their Gram-Stain Unknown (Skills Test 2). Introduction : 4 • The Gram staining • The Gram staining method, named after Hans Christian Gram. Report abuse.